To Cite:
Mirzaie Parsa
S, Soltani Banavandi
M J, Shahhosseiny
M H. Multiplex PCR Detection of blaCTX and blaSHV Resistance Genes in Escherchia coli Isolated from Milad Hospital in Tehran,
Hormozgan Med J.
2018
; 22(3):e87067.
doi: 10.5812/hmj.87067.
1.
Murray PR, Drew WL, Kobayashi GS, Thompson JH. Medical microbiology. (international student edition). USA: Wolfe Publishing; 1990. p. 103-7.
2.
Li Q, Lee JY, Castillo R, Hixon MS, Pujol C, Doppalapudi VR, et al. NB2001, a novel antibacterial agent with broad-spectrum activity and enhanced potency against beta-lactamase-producing strains. Antimicrob Agents Chemother. 2002;46(5):1262-8. doi: 10.1128/AAC.46.5.1262-1268.2002. [PubMed: 11959554]. [PubMed Central: PMC127132].
3.
Tenover FC, Raney PM, Williams PP, Rasheed JK, Biddle JW, Oliver A, et al. Evaluation of the NCCLS extended-spectrum beta-lactamase confirmation methods for Escherichia coli with isolates collected during Project ICARE. J Clin Microbiol. 2003;41(7):3142-6. doi: 10.1128/JCM.41.7.3142-3146.2003. [PubMed: 12843054]. [PubMed Central: PMC165309].
4.
Singh R, Saxena A, Singh H. Identification of group specific motifs in beta-lactamase family of proteins. J Biomed Sci. 2009;16:109. doi: 10.1186/1423-0127-16-109. [PubMed: 19954553]. [PubMed Central: PMC2793256].
5.
Winokur PL, Canton R, Casellas JM, Legakis N. Variations in the prevalence of strains expressing an extended-spectrum beta-lactamase phenotype and characterization of isolates from Europe, the Americas, and the Western Pacific region. Clin Infect Dis. 2001;32 Suppl 2:S94-103. doi: 10.1086/320182. [PubMed: 11320450].
6.
Fernandes R, Amador P, Oliveira C, Prudencio C. Molecular characterization of ESBL-producing Enterobacteriaceae in Northern Portugal. ScientificWorldJournal. 2014;2014:782897. doi: 10.1155/2014/782897. [PubMed: 24701189]. [PubMed Central: PMC3950362].
7.
Bajpai T, Pandey M, Varma M, Bhatambare GS. Prevalence of TEM, SHV, and CTX-M beta-lactamase genes in the urinary isolates of a tertiary care hospital. Avicenna J Med. 2017;7(1):12-6. doi: 10.4103/2231-0770.197508. [PubMed: 28182026]. [PubMed Central: PMC5255976].
8.
Asir J, Nair S, Devi S, Prashanth K, Saranathan R, Kanungo R. Simultaneous gut colonisation and infection by ESBL-producing Escherichia coli in hospitalised patients. Australas Med J. 2015;8(6):200-7. doi: 10.4066/AMJ.2015.2358. [PubMed: 26213583]. [PubMed Central: PMC4496720].
9.
Bush K, Jacoby GA, Medeiros AA. A functional classification scheme for beta-lactamases and its correlation with molecular structure. Antimicrob Agents Chemother. 1995;39(6):1211-33. [PubMed: 7574506]. [PubMed Central: PMC162717].
10.
Bush K. The ABCD's of beta-lactamase nomenclature. J Infect Chemother. 2013;19(4):549-59. doi: 10.1007/s10156-013-0640-7. [PubMed: 23828655].
11.
Liakopoulos A, Mevius D, Ceccarelli D. A review of SHV extended-spectrum beta-lactamases: Neglected yet ubiquitous. Front Microbiol. 2016;7:1374. doi: 10.3389/fmicb.2016.01374. [PubMed: 27656166]. [PubMed Central: PMC5011133].
12.
Al-Agamy MH, Shibl AM, Tawfik AF. Prevalence and molecular characterization of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in Riyadh, Saudi Arabia. Ann Saudi Med. 2009;29(4):253-7. doi: 10.4103/0256-4947.55306. [PubMed: 19587523]. [PubMed Central: PMC2841451].
13.
Krishnamurthy V, G SV, Kumar MS, H VP, R P, E RN. Phenotypic and genotypic methods for detection of extended spectrum beta lactamase producing Escherichia coli and Klebsiella pneumoniae Isolated from ventilator associated pneumonia. J Clin Diagn Res. 2013;7(9):1975-8. doi: 10.7860/JCDR/2013/6544.3376. [PubMed: 24179913]. [PubMed Central: PMC3809652].
14.
Li Y, Zheng B, Li Y, Zhu S, Xue F, Liu J. Antimicrobial susceptibility and molecular mechanisms of fosfomycin resistance in clinical Escherichia coli isolates in Mainland China. PLoS One. 2015;10(8). e0135269. doi: 10.1371/journal.pone.0135269. [PubMed: 26252888]. [PubMed Central: PMC4529152].
15.
Shahcheraghi F, Nasiri S, Noveiri H. [Detection of bla TEM and bla SHVgenes among clinical isolates of E. coli from Tehran hospitals]. Iran J Med Microbiol. 2007;1(3):1-8. Persian.
16.
Ehlers MM, Veldsman C, Makgotlho EP, Dove MG, Hoosen AA, Kock MM. Detection of blaSHV, blaTEM and blaCTX-M antibiotic resistance genes in randomly selected bacterial pathogens from the Steve Biko Academic Hospital. FEMS Immunol Med Microbiol. 2009;56(3):191-6. doi: 10.1111/j.1574-695X.2009.00564.x. [PubMed: 19453751].
17.
Branger C, Zamfir O, Geoffroy S, Laurans G, Arlet G, Thien HV, et al. Genetic background of Escherichia coli and extended-spectrum beta-lactamase type. Emerg Infect Dis. 2005;11(1):54-61. doi: 10.3201/eid1101.040257. [PubMed: 15705323]. [PubMed Central: PMC3294364].
18.
Dhillon RH, Clark J. ESBLs: A clear and present danger? Crit Care Res Pract. 2012;2012:625170. doi: 10.1155/2012/625170. [PubMed: 21766013]. [PubMed Central: PMC3135063].
20.
Reist M, Geser N, Hachler H, Scharrer S, Stephan R. ESBL-producing Enterobacteriaceae: Occurrence, risk factors for fecal carriage and strain traits in the Swiss slaughter cattle population younger than 2 years sampled at abattoir level. PLoS One. 2013;8(8). e71725. doi: 10.1371/journal.pone.0071725. [PubMed: 23977126]. [PubMed Central: PMC3748101].
21.
Pagan-Rodriguez D, Zhou X, Simmons R, Bethel CR, Hujer AM, Helfand MS, et al. Tazobactam inactivation of SHV-1 and the inhibitor-resistant Ser130 -->Gly SHV-1 beta-lactamase: Insights into the mechanism of inhibition. J Biol Chem. 2004;279(19):19494-501. doi: 10.1074/jbc.M311669200. [PubMed: 14757767].
22.
Mobasher Kare Jeddi A, Nahaei M, Mobayyen H, Pornour M, Sadeghi J. [Molecular study of extended-spectrum beta-lactamase (SHVtype) in Esherichia coliand Klebsiella pneumoniae isolated from Medical Centers of Tabriz]. Iran J Med Microbiol. 2009;2(3):9-17. Persian.
24.
Lucet JC, Chevret S, Decre D, Vanjak D, Macrez A, Bedos JP, et al. Outbreak of multiply resistant enterobacteriaceae in an intensive care unit: Epidemiology and risk factors for acquisition. Clin Infect Dis. 1996;22(3):430-6. doi: 10.1093/clinids/22.3.430. [PubMed: 8852958].
25.
Li S, Qu Y, Hu D, Shi YX. Comparison of extended spectrum beta-lactamases-producing Escherichia coli with non-ESBLs-producing E. coli: Drug-resistance and virulence. World J Emerg Med. 2012;3(3):208-12. doi: 10.5847/wjem.j.1920-8642.2012.03.009. [PubMed: 25215065]. [PubMed Central: PMC4129785].
26.
Canton R, Coque TM. The CTX-M beta-lactamase pandemic. Curr Opin Microbiol. 2006;9(5):466-75. doi: 10.1016/j.mib.2006.08.011. [PubMed: 16942899].
27.
Mendonca N, Manageiro V, Bonnet R, Canica M. Biochemical characterization of SHV-55, an extended-spectrum class A beta-lactamase from Klebsiella pneumoniae. Antimicrob Agents Chemother. 2008;52(5):1897-8. doi: 10.1128/AAC.01525-07. [PubMed: 18316524]. [PubMed Central: PMC2346664].
28.
Arpin C, Labia R, Andre C, Frigo C, El Harrif Z, Quentin C. SHV-16, a beta-lactamase with a pentapeptide duplication in the omega loop. Antimicrob Agents Chemother. 2001;45(9):2480-5. doi: 10.1128/AAC.45.9.2480-2485.2001. [PubMed: 11502518]. [PubMed Central: PMC90681].
29.
Bonnet R. Growing group of extended-spectrum beta-lactamases: the CTX-M enzymes. Antimicrob Agents Chemother. 2004;48(1):1-14. doi: 10.1128/AAC.48.1.1-14.2004. [PubMed: 14693512]. [PubMed Central: PMC310187].
30.
Monstein HJ, Ostholm-Balkhed A, Nilsson MV, Nilsson M, Dornbusch K, Nilsson LE. Multiplex PCR amplification assay for the detection of blaSHV, blaTEM and blaCTX-M genes in Enterobacteriaceae. APMIS. 2007;115(12):1400-8. doi: 10.1111/j.1600-0463.2007.00722.x. [PubMed: 18184411].
31.
Fallah F, Hakemi VM, Hashemi A, Shams S. [Emergence of novel plasmid-mediated Beta-lactamase in Klebsiella pneumonia]. Qom Univ Med Sci J. 2013;6(4):104-16. Persian.
32.
Meyer E, Gastmeier P, Schwab F. The burden of multiresistant bacteria in German intensive care units. J Antimicrob Chemother. 2008;62(6):1474-6. doi: 10.1093/jac/dkn391. [PubMed: 18794158].
33.
Ho PL, Wong RC, Chow KH, Yip K, Wong SS, Que TL. CTX-M type beta-lactamases among fecal Escherichia coli and Klebsiella pneumoniae isolates in non-hospitalized children and adults. J Microbiol Immunol Infect. 2008;41(5):428-32. [PubMed: 19122926].
34.
Yazdi M, Nazemi A, Miri Nargesi MS, Khataminejad MR, Sharifi SA, Babaei Koochaksarai M. [The prevalence of beta-lactamase resistance genes TEM/CTX-M/SHV in Escherichia coli isolated from urinary tract infection in Tehran]. J Lab Sci. 2010;4(1):48-54. Persian.
35.
Bali EB, Accedil L, Sultan N. Phenotypic and molecular characterization of SHV, TEM, CTX-M and extended-spectrum-lactamase produced by Escherichia coli, Acinobacter baumannii and Klebsiella isolates in a Turkish hospital. Afr J Microbiol Res. 2010;4(8):650-4.
36.
Soltan Dallal MM, Mobasseri G, Fallah Mehrabadi J, Eshraghian MR, Rastegar Lari A, Molla Aghamirzaei H, et al. Detection of CTX-M-1 beta lactamase gene in Escherichia coli isolated from clinical samples by polymerase chain reaction (PCR). Tehran Univ Med J. 2011;69(1):16-21.
37.
Altinkum SM, Ergin S, Bahar H, Torun MM. CTX-M-15 type extended spectrum β-lactamase producing Escherichia coli and Klebsiella pneumoniae: A developing problem in infected outpatients and hospitalised patients in Istanbul, Turkey. Afr J Microbiol Res. 2013;7(8):692-7.
38.
Miraalami G, Parviz M, Khalajzadeh S. Evaluation of antibiotic resistance in extended-spectrum beta- lactamase (ESBL) genes in the E. coli isolates of urinary infections. J Babol Univ Med Sci. 2015;17(8):19-26.
39.
Hamdy Mohammed el S, Elsadek Fakhr A, Mohammed El Sayed H, Al Johery SA, Abdel Ghani Hassanein W. Spread of TEM, VIM, SHV, and CTX-M beta-lactamases in imipenem-resistant gram-negative bacilli isolated from Egyptian hospitals. Int J Microbiol. 2016;2016:8382605. doi: 10.1155/2016/8382605. [PubMed: 27123005]. [PubMed Central: PMC4830709].
40.
Thomson KS. Controversies about extended-spectrum and AmpC beta-lactamases. Emerg Infect Dis. 2001;7(2):333-6. doi: 10.3201/eid0702.700333. [PubMed: 11294735]. [PubMed Central: PMC2631719].
41.
Pitout JD, Laupland KB. Extended-spectrum beta-lactamase-producing Enterobacteriaceae: An emerging public-health concern. Lancet Infect Dis. 2008;8(3):159-66. doi: 10.1016/S1473-3099(08)70041-0. [PubMed: 18291338].
Readers' Comments