The effect of endurance training on dynein motor protein expression in Wistar male rats sciatic nerves with diabetic neuropathy
Hormozgan Medical Journal: January 01, 2018, 21 (1); e87263
February 24, 2014
Article Type: Research Article
November 12, 2013
February 24, 2014
A , Gharakhanlou
R , Kordi
M R , Movahedin
M , Jahani Golbar
S, et al. The effect of endurance training on dynein motor protein expression in Wistar male rats sciatic nerves with diabetic neuropathy,
Hormozgan Med J.
Introduction: Most neurodegenerative diseases are associated with the disruption of
axonal transport and this might also be related to diabetes-associated disorders affecting
the nervous system. Cytoplasmic dynein is a very important motor driving the movement
of a wide range of cargoes toward the minus ends of microtubules. The effects of
endurance training on dynein motor protein expression in Wistar male rats sciatic nerves
with diabetic neuropathy were investigated.
Methods: Twenty one male Wistar rats were divided into three groups: diabetic control
(DC: n=7), diabetic trained (DT: n=7) and Healthy control (HC: n=7). Two weeks post
STZ injection (45 mg/kg, i.p.), rats were subjected to treadmill exercise for 5 days a week
for over 6 weeks. Levels of mRNA and protein content were determined by Real time-
PCR and ELISA.
Results: The blood glucose levels and dynein mRNA in both sensory and motor parts
and dynein content in sciatic nerves were increased by diabetes (P≤0.05). Furthermore,
exercise training modulated the dynein mRNA and dynein content to normal levels in
diabetic trained rats (P≤0.05). Moreover, exercise training reduced blood glucose levels
in the DT rats (P≤0.05).
Conclusion: The results of the present study confirm that dynein expresses up-regulation
in STZ-induced diabetic rats. Increase in the amount of dynein expression can be a
compensatory response in the lower trophic support and burden imposed on autophagy
system. These alterations suggest that retrograde axonal transport may be impaired in
diabetic rats. In addition, endurance training as a non-pharmacotherapy strategy can
modulate these alterations.
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