The relationship between blastocystis hominis infection and Irritable Bowel Syndrome (IBS) and comparing direct wet mount, stool culture, Formalin- Ether and trichrome staining procedures for identifying organisms


Nashmin Mohemmi 1 , Mohammad Moradi 1 , Alireza Khalilian 2 , Amir Hossein Maghsood 3 , Mohammad Fallah 4 , *


1 MSc of Parasitology, Hamadan University of Medical Sciences, Hamadan, Iran.

2 Assistant Professor Department of Internal Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.

3 Associate Professor Department of Parasitology, Hamadan University of Medical Sciences, Hamadan, Iran.

4 Professor Department of Parasitology, Hamadan University of Medical Sciences, Hamadan, Iran.


Hormozgan Medical Journal: 19 (2); e87432
Published Online: February 01, 2014
Article Type: Research Article
Received: June 17, 2013
Accepted: February 01, 2014




Introduction: Blastocystis hominis is a parasite found in the stools of human as well as
in the stools of other animals and recently its pathogenic potential has been increasingly
recognized. Irritable Bowel Syndrome (IBS) is a digestive system’s disorder which is
featured by change in bowel habits, abdominal pains and other symptoms similar to
Blastocystis hominis infection. There is little information about this syndrome and its
etiology is still unknown. This study aims at analyzing the relationship between
Blastocystis hominis and IBS.
Methods: In a case-control study, a total of 81 patients with IBS and 81 patients with
other digestive disorders, but without IBS, who had referred to Shahid Beheshti Hospital
in Hamedan City, were selected. The disease was diagnosed by a gastroenterologist in
terms of the relevant criteria. Blastocystis hominis infection in this study was diagnosed
using stool test through four techniques: direct wet mount, stool culture, formalin-ether
concentration and trichrome staining.
Results: Totally, 43 women (53%) and 38 men (47%), in the age range of 13 to 64 years
old, participated in this study. Using the stool culture technique, as the golden standard,
infection in 37 members (45.67%) of the case group and 18 members (22.22%) of the
control group was reported, which this difference was significant, statistically (P<0.001).
There was difference between case and control groups in terms of Blastocystis infection
with all four techniques.
Conclusion: The results of this study indicated that since the infection rate in the case
group was higher than that in the control group, a relationship between Blastocystis
hominis infection and IBS is possible. Speaking about infection diagnosis, culture
technique was more sensitive than other techniques used to diagnose Blastocystis hominis


Blastocystis Hominis - Irritable Bowel Syndrome – Parasites

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