The effect of Hydro-alcoholic extract of pumpkin seeds on Oogenesis and Hormone-Pituitary-Ovarian Axis in Mature Rats

AUTHORS

Samaneh Motamed Jahromi 1 , Vahid Hemayatkhah Jahromi 2 , * , Habiballah Johari 3

1 MSc of Physiology, Jahrom University of Medical Sciences, Jahrom, Iran.

2 Assistant Professor Department of Biology, Islamic Azad University Jahrom Branch, Jahrom, Iran.

3 Assistant Professor Department of Physiology, Islamic Azad University Darab Branch, Darab, Iran.

How to Cite: Motamed Jahromi S , Hemayatkhah Jahromi V , Johari H . The effect of Hydro-alcoholic extract of pumpkin seeds on Oogenesis and Hormone-Pituitary-Ovarian Axis in Mature Rats, Hormozgan Med J. 2015 ; 19(2):e87439.

ARTICLE INFORMATION

Hormozgan Medical Journal: 19 (2); e87439
Published Online: January 11, 2014
Article Type: Research Article
Received: July 14, 2013
Accepted: January 11, 2014

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Abstract

Introduction: In traditional medicine, pumpkin seeds are prescribed and used commonly
as energetic and sexual enhancer substances. This study was planned and carried out in
order to fill the gap caused by the lack of sufficient scientific evidence on the effect of this
substance on oogenesis and hormone-pituitary-ovarian axis. Revealing the effect of hydroalcoholic
extract of pumpkin seeds on oogenesis and hormone-pituitary-ovarian axis of
mature rats.
Methods: In this experimental study a number of 30 mature female Wistar rats (weight
range: 180±10) were tested. They were divided randomly into four 6-member groups,
including three experiment and a control groups. Members of experimental groups were
injected intraperitoneally by the extract (with 50, 100, and 200 mg/kg of hydro-alcoholic
extract of pumpkin seeds) for 21 consecutive days. A day after the last injection, the rats'
blood was taken for analyzing the sexual hormones and ovaries were dissected for
histological studies.
Results: Injecting hydro-alcoholic extract of pumpkin seeds increases significantly the
serum level of estrogen, progesterone, LH, FSH, number of graafian follicles and also
decreases significantly the left ovaries' weight and number of primordial, primary, and
secondary follicles in experimental groups in contrast to control group (P<0.05).
Conclusion: Injecting hydro-alcoholic extract of pumpkin seeds increases significantly
the oogenesis procedure, LH, FSH, estrogen and progesterone levels.

Keywords

Oogenesis – LH – LH – Esterogens – Progestron

© 2015, Hormozgan Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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