Antioxidant properties of hydro-alcoholic extract and extract of nepeta crispa in Lorestan province

AUTHORS

Hasan Ahmadvand 1 , * , Hamzeh Amiri 2 , Hamid Dalvand 3

1 Associate Professor Department of Biochemistry, Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khoramabad, Iran.

2 Associate Professor Department of Biology, Lorestan University, Khoramabad, Iran.

3 Department of Biology, Islamic Azad University, Brojerd Branch, Borojerd, Iran.

How to Cite: Ahmadvand H , Amiri H , Dalvand H . Antioxidant properties of hydro-alcoholic extract and extract of nepeta crispa in Lorestan province, Hormozgan Med J. 2015 ; 19(3):e87471.

ARTICLE INFORMATION

Hormozgan Medical Journal: 19 (3); e87471
Published Online: November 11, 2013
Article Type: Research Article
Received: January 10, 2013
Accepted: November 11, 2013

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Abstract

Introduction: Antioxidants protect body against the oxidative stress caused by free
radicals. This study aims at comparing the antioxidant properties of hydro-alcoholic
extract and extract of Nepeta crispa (from its shoot part).
Methods: The study was carried out in Lorestan University of Medical Sciences in 2012.
Both hydro-alcoholic extract and essence of Nepeta crispa were prepared and antioxidant
properties of samples were assessed using different methods. The ability to inhibit free
radicals production was measured through 2, 2-diphenyl-1-picrylhydrazyl (DPPH)
method. Total antioxidant capacity was measured using Phosphomolybdate Method.
Likewise, phenol and flavonoid compounds of samples were measured using folinciocalteu
Zishen method.
Results: The results showed that the total antioxidant capacity of hydro-alcoholic extract
and essence of Nepeta crispa were equal to 0.23±0.12 and 0.82±0.40 Nmol ascorbic acid
per gram extract or essence. The phenol content of hydro-alcoholic extract and essence of
Nepeta crispa were 254.63±15.75 and 6.94±1.37 mg gallic acid per gram extract or
essence, respectively. Flavonoid content of hydro-alcoholic extract and essence of Nepeta
crispa were equal to 5.53±0.34 and 0.36±0.06 mg quercetin per gram extract or essence,
respectively. IC50 value (concentration that will inhibit 50% of free radicals production),
hydro-alcoholic extract, essence of plant and Butylated hydroxytoluene (BHT), as positive
control, were equal to 2.09±0.11, 6.05, and 3.88±1 μg/ml, respectively.
Conclusion: The results showed that the hydro-alcoholic extract has better antioxidant
properties in contrast to essence of Nepeta crispa. As a result, hydro-alcoholic extract of
Nepeta crispa is a good and available source of antioxidant compounds which can be
used, if useful, in food, pharmaceutical and industrial products.

Keywords

Essential Oil – Antioxidants – Nepeta Crispa

© 2015, Hormozgan Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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