The effect of interference of morphine and immobility stress on performance of pituitary–adrenal axis in mature male rats

AUTHORS

Seyed Ebrahim Hosseini 1 , * , Mojhdeh Heidari 2

1 Associate Professor Department of Biology, Islamic Azad University Sciences and Research Branch, Fars, Iran.

2 MSc of Biology, Farhangiyan University, Shiraz, Iran.

How to Cite: Hosseini S E, Heidari M. The effect of interference of morphine and immobility stress on performance of pituitary–adrenal axis in mature male rats, Hormozgan Med J. 2014 ; 18(1):e87837.

ARTICLE INFORMATION

Hormozgan Medical Journal: 18 (1); e87837
Published Online: June 11, 2013
Article Type: Research Article
Received: September 18, 2012
Accepted: June 11, 2013

Crossmark

CHEKING

READ FULL TEXT
Abstract

Introduction: Morphine as an alkaloid from the poppy plant (papaver somniferum) is extracted and used by millions of people around the world and also types of stresses threated such as immobility stress the health of many people. The aim of this study was to investigate the interference of morphine and immobility stress on plasma levels of hormones of ACTH and corticosterone in mature male rats.
Methods: In this empirical study, 70 mature male Wistar rats were enrolled as the control, sham and experiment groups, randomly. 3 experimental groups of different types received doses of 10, 20 and 30 mg/kgBW morphine, and 2 experimental group, one received for 2 hours immobility stress and other for 2 hours immobility stress and other plus morphine with doses of 30 mg/kgBW and the sham group received 1cc of saline intraperitoneally for 5 days. 24 hours after the last injection, mice were bled from the heart and hormones of ACTH and corticosterone levels were measured. The data were evaluated using ANOVA and Tuky test. Results: The results showed that the maximum dose of morphine and immobility stress increases plasma levels of ACTH and corticosterone hormones (P<0.001) and simultaneous administration of morphine and immobilization stress enhances the effect of each alone.
Conclusion: Morphine and immobility stress intensify their effect on hormones of ACTH and corticosterone. Therefore, further investigation can discover on the role of stress and morphine on disease incidence and disease prevention, in particular psychosomatic in human specimens.

Keywords

ACTH – Corticosterone – Morphine - Rats

© 2014, Hormozgan Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

Fulltext

References

  • 1.

    References are included in the PDF.

  • COMMENTS

    LEAVE A COMMENT HERE: