Evaluation of physiochemical properties of curcumin-loaded Chitosan Tripolyphosphate Nanoparticles


Mirza Ali Mofazzali-Jahromi 1 , Majid Pirestani 2 , Sharafaldin Al-Musawi 3 , Mehdi Fasihi-Rammandi 4 , Kazem Ahmadi 5 , Zuhair Mohammad Hassan 6 , Mahdi Kamali 7 , Hajar Rajayi 8 , Reza Mirnejad 9 , *


1 PhD of Immunology , Molecular Biology Research Center, Baqiyatallah University of Medical Sciences

2 Assistant Professor Department of Parasitology , Tarbiat Modares University

3 PhD of Nanobiotechnology , Tarbiat Modares University

4 Assistant Professor Department of Biotechnology , Molecular Biology Research Center, Baqiyatallah University of Medical Sciences

5 Professor Department of Immunology , Baqiyatallah University of Medical Sciences

6 Professor Department of Immunology , Tarbiat Modares University,

7 MSc of Immunology, Tarbiat Modares University

8 Assistant Professor Department of Nanobiotechnology , Nanobiotechnology Research Center , Baqiyatallah University of Medical Sciences

9 Associate Professor Department of Medical Bachteriology , Molecular Biology Research Center , Baqiyatallah University of Medical Sciences, Tehran, Iran.


Hormozgan Medical Journal: 20 (1); e87916
Published Online: June 30, 2014
Article Type: Research Article
Received: February 23, 2014
Accepted: June 30, 2014




Introduction: Curcumin is a water-insoluble agent with some anticancer and antimicrobial effects; while, solubility is essential for a medicine to reach target cells. This study set out to use chitosan tripolyphosphate nanoparticles (CTN) for making curcumin water-soluble, and delivering the required dosage of it to target cells.

Methods: In this study, the nanoparticles were fabricated from chitosan and tripolyphosphate using the ionotropic gelation method. The emulsified solution of curcumin was also loaded during the fabrication of CTNs. Then, the size and loaded amount of the nanoparticles were measured with a zeta potential analyzer; in addition, the shape and size of them were evaluated through atomic-force microscopy. Moreover, the amount of drug loaded chitosan nanoparticles was measured, using a spectrophotometer.

Results: According to zeta analyzer results, the size and charge of the curcumin-contained chitosan tripolyphosphate nanoparticles were 165±5 nm and +7±2 mV, respectively. According to the AFM findings, the obtained nanoparticles had spherical shape. Spectrophotometer showed that the curcumin loaded into tripolyphosphate nanoparticles was 67±1%.

Conclusion: Our study suggests that a significant amount of water-soluble curcumin can be loaded into CTNs using ionotropic gelation method.


Nanosystem Nanoparticles Curcumin

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