Antifungal effect of essential oil of Bunium persicum on Candida albicans, In vitro study


Ali Rashidi 1 , Seyed Amir Ali Mahbod 2 , Alireza Shahb Jahanloo 3 , * , Ayda Gholami 1 , Mehregan Heydari Hengami 4


1 Medical Student, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

2 Associate Professor Department of Mycology, Artesh University of Medical Sciences, Tehran, Iran.

3 Assistant Professor Department of Community Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

4 BSc of Microbiology, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.


Hormozgan Medical Journal: 17 (1); e87921
Published Online: July 23, 2012
Article Type: Research Article
Received: February 27, 2012
Accepted: July 23, 2012




Introduction: Bunium persicum is a gramine plant from Umbelliferae family. Candida
albicans is a kind of yeast known as normal flora of skin and gastrointestinal tract (GIT),
respiratory and genitourinary (GU) mucosa. This fungi makes disease in immunodeficient
individuals. The aim of this study was to assess the antifungal effect of essential oil of
Bunium persicum on Candida albicans.
Methods: 28 plate's receptacle of Candida albicans were passaged on Sabouraud dextrose
agar medium. Seven plates were assign as control (without essential oil of Bunium
persicum) and 21 plates as cases, consisted 1mg/ml, 2 mg/ml and 3mg/ml (7 plates for each
of them) Bunium persicum essential. All plates incubated in 37°C incubator for 72 hours.
Colony counting was done each 24h by crossbar paper and Heerbrugg WILD stereoscope.
Finally, descriptive statistics was used for results presentation.
Results: After the first 24hr, Candida albicans yeasts had growth in all case plates but no
growth was seen in control plates. In the second 24hr, all control and 1mg/ml essential oil
of Bunium persicum plates, had growth, but no culture observed in 2mg/ml and 3mg/ml
essential oil of Bunium persicum plates. Finally after 72hr, although culture was seen in
control (Innumerable), 1mg/ml (Innumerable) and 2mg/ml (Average=46 colony) essential
oil of Bunium persicum plates, no culture was observed in 3 mg/ml plates.
Conclusion: Results of present study demonstrated that, all 3 concentrations could delay
culture of Candida albicans and induce stay effect on that. Time of staying culture of fungi
was related to essential concentration in medium.


Black Cumin - Candida Albicans - In vitro

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