Monocyte isolation using serum dextrose 5% with autologous plasma

AUTHORS

Elham Beiranvand 1 , saeed Abediankenari 2 , * , behnoosh Beiranvand 3 , hadi Hasannia 4

AUTHORS INFORMATION

1 MSc of Microbiology,Mazandaran University of Medical Sciences, Sari, Iran

2 Associate Professor Department of Immunology,Mazandaran University of Medical Sciences, Sari, Iran.

3 General Practioner,Ahvaz Jondiahapour University of Medical Sciences, Ahvaz, Iran.

4 MSc of Immunology,Mazandaran University of Medical Sciences, Sari, Iran.

ARTICLE INFORMATION

Hormozgan Medical Journal: 16 (6); e88040
Published Online: April 23, 2012
Article Type: Research Article
Received: June 01, 2011
Accepted: April 23, 2012

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Abstract

Introduction: Mammalian cells culture is an important method for producing recombinant proteins, monoclonal antibody, and hormones application. One of the common methods in the monocyte isolation is using flask adherent cell culture in RPMI-1640 medium. We aimed, in this study, cell culture medium using dextrose 5% with autologous plasma to produce inexpensive medium for isolation of monocytes. Methods: In this experimental study, we isolated peripheral blood mononuclear cells from 40 healthy volunteers, the isolated cells were cultured in RPMI 1640 medium containing 10% fetal calf serum and in the media supplemented serum dexterous 5%containing autologous plasma under 37°C and 5% CO2 in a cell incubator. Then, count, viability and morphological cells were surveyed by trypan blue and invert microscope. Results: We isolated 1.8- 2× 106 cells /ml of RPMI culture media and 2- 2.2×106 /ml macrophage of serum dexterous 5% containing autologous plasma. There was not a significant difference between two methods (P>0.05). Also, we did not see any morphological variation in two culture media. Conclusion: Our results showed that dextrose 5% can be used in monocyte isolation from peripheral blood mononuclear cells

Keywords

Cell Culture – Serum – Monocytes

© 2013, Hormozgan Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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