Correlation between histopathological criteria of Human Papilloma Virus infection and the presence of HPV in paraffin-embedded cervical biopsies based on PCR assay

AUTHORS

Hamidreza Ghasemian Moghadam 1 , Hossein Ayatollahi 2 , Seyed Alireza Sobhani 3 , * , Zahra Etaati 4 , Shahram Zare 5

1 Resident of Pathology, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

2 Associate Professor Department of Pathology, Mashhad University of Medical Sciences, Mashhad, Iran.

3 Assistant Professor Department of Pathology, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

4 Assistant Professor Department of Obstetrics and Gynecology, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

5 Associatr Professor Department of Community Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

How to Cite: Ghasemian Moghadam H, Ayatollahi H, Sobhani S A, Etaati Z, Zare S . Correlation between histopathological criteria of Human Papilloma Virus infection and the presence of HPV in paraffin-embedded cervical biopsies based on PCR assay, Hormozgan Med J. 2013 ; 17(3):e88129.

ARTICLE INFORMATION

Hormozgan Medical Journal: 17 (3); e88129
Published Online: April 30, 2013
Article Type: Research Article
Received: March 23, 2012
Accepted: April 30, 2013

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Abstract

Introduction: This study was carried out to correlate histological findings in cervical
lesions to human papilloma virus (HPV), as detected by polymerase chain reaction (PCR).
Methods: In this descriptive study, out of 143 cervical biopsies, 50 biopsies, containing
sufficient histopathologic criteria, were examined. PCR assay was performed with the
primers GP05/06+ and, as control, the beta-globin gene was amplified. The morphological
findings were correlated to HPV positivity: koilocytic atypia, multinucleation, acanthosis,
papillomatosis, dyskeratotic cell, mitosis in the lower basal third of the epithelium,
hyperplasia of basal layers.
Results: In 50 selected samples pathologic diagnosis was chronic cervicitis in 37 cases
(74%) and cervical intraepithelial neoplasia (CIN) in 13 cases (including CIN I in 6 cases,
CIN II in 5 cases, CIN III in 2 cases). HPV DNA was positive in 6 cases, including 5.4%
of chronic cervicitis (2 cases) and 30.8% of CINs (including 33.3% of CIN I, 40% of CIN
II). The analysis did not indicate any strong relationship between morphological criteria and
HPV. The only finding showed an independent, straight and weak correlation between HPV
and koilocytic atypia (P value=0.003 & Kappa=0.296) with sensitivity of 100% and a
relatively low specificity of 63.6%. By excluding mild koilocytic atypia, this correlation was
amplified (P<0.001 & Kappa=0.669) and appear acceptable sensitivity of 83.3% and
specificity of 93.2%. The finding with highest positive and negative predictive value was the
presence of definite koilocytosis (PPV=62.5% & NPV=97.6%).
Conclusion: In spite of association of some HPV infection with chronic cervicitis; there is
no correlation between severity of chronic cervicitis and HPV infection. Although
koilocytosis is a good indicative criterion for HPV infection; nevertheless, there is some
limitations in histological diagnosis of cervical HPV infection.

Keywords

Human Papilloma Virus (HPV) - Polymerase Chain Reaction (PCR) - Biopsy

© 2013, Hormozgan Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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