Genetic mutations in 57 and 58 codons gene of Plasmodium vivax dihydrofolate reductase

AUTHORS

Abbas Shahbazi 1 , Jalal Zaman 2 , * , Mohammad Asgharzadeh 3 , Adel Spotin 4 , Sajad Jeddi 2

1 Associate Professor Department of Prasitology, Infections and Tropical Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

2 PhD Student of Molecular Medicine, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

3 Associate Professor Department of Biotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.

4 PhD Student of Parasitology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

How to Cite: Shahbazi A, Zaman J, Asgharzadeh M, Spotin A, Jeddi S. Genetic mutations in 57 and 58 codons gene of Plasmodium vivax dihydrofolate reductase, Hormozgan Med J. 2013 ; 17(5):e88196.

ARTICLE INFORMATION

Hormozgan Medical Journal: 17 (5); e88196
Published Online: October 10, 2012
Article Type: Research Article
Received: May 18, 2012
Accepted: October 10, 2012

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Abstract

Introduction: The use of Sulfadoxine and pyrimethamine (SP) for treatment of vivax
malaria is not common in most of malarious areas because of sensivity of this parasite to
chloroquine. But, Plasmodium vivax isolates are exposed to SP because of mixed infection
with P.falciparum and this subject has lead to emergence of mutations in P.vdhfr gene. As
Plasmodium vivax is the most prevalent species of human malaria parasites in Iran,
monitoring of resistance of the parasite against the drug would be necessary.
Methods: In this study, 50 blood samples of symptomatic patients were collected from four
separated geographical regions of south-east of Iran. Point mutations at residues 57, 58 were
detected by PCR-RFLP method.
Results: Polymorphism at positions 58R, of Pvdhfr gene has been found in 12% of isolates
and mutation at residue F57 was not detected. Alleles with two points mutations in Pvdhfr
gene were not found.
Conclusion: This study showed that P. vivax in Iran is under the pressure of SP and the
sensitivity level of the parasite to SP determines that this subject can lead to emergence of
influence mutations in increase of drug resistance. So, this fact must be considered in
development of malaria control program.

Keywords

Plasmodium Vivax - Sulfadoxine - Pyrimethamine – Dihydrofolate Reductase - Drug Reresistance

© 2013, Hormozgan Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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