Investigating the rate of glutathione S-transferase T1 and M1 genes deletion in patients with lung cancer

AUTHORS

Bibi Fatemeh Haghirasadat 1 , Tahereh Nazari 2 , Meysam Omidi 1 , Mostafa Azimzadeh 1 , Mohammadhasan Sheikhha 3 , *

AUTHORS INFORMATION

1 PhD Student of Nanobiotechnology, Tehran University, Tehran, Iran.

2 MSc Student of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

3 Associate Professor Department of Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

ARTICLE INFORMATION

Hormozgan Medical Journal: 17 (5); e88198
Published Online: May 31, 2012
Article Type: Research Article
Received: January 28, 2012
Accepted: May 31, 2012

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Abstract

Introduction: Glutathione S-tarnsferases (GST) gene family is one of the enzymes which
is responsible for detoxifying mutagens and Carcinogens chemicals. People with null
genotype of GSTM1 and GSTT1 genes are at higher risk of developing cancer, especially
those who are related to smoking. The goal of this study was investigating the rate of
glutathione S-transferase T1 and M1 genes deletion and their role in patients with lung
cancer.
Methods: In this case-control study, DNA extraction with salting out method was carried
out upon 70 healthy controls and 30 patients with lung cancer. For determining deletions, a
multiplex PCR was performed for GSTT1, GSTM1 and beta-globin as a control gene.
Statistical analysis was done by Pearson's chi-square test and Fisher’s exact test.
Results: Out of 30 patients and 70 controls: 3 (10%) cases and one (1.4%) control were
double null genotype; while in 19 (63.3%) patients and 59 (84.3%) controls, none of the
genes were deleted. In 5 (16.7%) cases and 5 (7.1%) controls GSTT1 were deleted and in 9
(30%) cases and 7 (10%) controls GSTM1 was deleted. Statistical analysis showed that
GSTM1 deletion is significantly higher in patients in comparison to the controls (P=0.018).
Deletion of at least one of the GSTM1 or GSTT1 genes was significantly higher in patients
group compar to the controls (P=0.033).
Conclusion: Deletion in glutathione S-transferse genes was related to detoxifying
carcinogenes and this could have been related to the development of lung cancer; and in
people with at least one deletion or both, the risk of lung cancer was more (especially
GSTM1) comparing with those who have two wild type genotypes of these two genes.

Keywords

glutathione S-transferse - GSTM1 - GSTT1 - Lung Cancer

© 2013, Hormozgan Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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