Comparison of antimicrobial effect of washing solutions of root canals on extracted teeth

AUTHORS

Zohreh Ahangari 1 , * , Mohammad Samieei 2 , Mohammadamin Yalme 3 , Hedyeh Koohsedghi 4

1 Associated Professor, Department of Endodontics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

2 Resident, Department of Endodontics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

3 Endodontist, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

4 Dentist, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

How to Cite: Ahangari Z, Samieei M, Yalme M, Koohsedghi H. Comparison of antimicrobial effect of washing solutions of root canals on extracted teeth, Hormozgan Med J. 2009 ; 13(2):e88643.

ARTICLE INFORMATION

Hormozgan Medical Journal: 13 (2); e88643
Published Online: January 11, 2009
Article Type: Research Article
Received: May 29, 2008
Accepted: January 11, 2009

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Abstract

Introduction: The aim of the study was to compare the antimicrobial effects of
2.5% sodium hypochlorite (NaOCI), 2% chlorhexidine Gloconate (CHX) and
Biopure MTAD (MATD) on Enterococcus (E) faecalis that contaminated root
canals of extracted teeth.
Methods: 70 intact extracted single-rooted teeth with straight root-canal randomly
were divided into 5 groups as positive control, negative control, 2.5% NaOCI, CHX
and MTAD groups. Each tooth was instrumented using the passive step-back technique
hand and rotary instruments. Entrococcus faecal was incubated into the canals and
grew for 4 weeks. Canals were irrigated using three mentioned solutions for 5 minutes.
Samples were taken from canal walls and transferred into Brain Heart Infusion (BHI)
culture medium and placed in an incubator qt 370C for 96 hours and then
bacteriological evaluations were done. Kruscall-Wallis test was used by means of SPSS
software, for the statistical analysis.
Results: Bacterial growth was observed in only one sample of MTAD group
(5%), in 4 of CHX group (20%) and 5 of NaOCI group (25%). Statistical
differences among the groups were not significant.
Conclusion: It seems that all three solutions have acceptable antimicrobial effect on
Entrococcus faecal.

Keywords

Root Canal Irrigants - Entrococcus Faecal – MTAD – Sodium Hypochlorite – Chlorhexidine

© 2009, Hormozgan Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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