Validation of microscopic diagnosis of malaria in field laboratories of malarious areas of Iran by Nested PCR

AUTHORS

Abbas Shahbazi 1 , * , Ahmad Raeisi 2 , Hossein Mirhendi 3 , Mohammad Asgharzadeh 4 , Homayoun Sadeghi Bazargani 5

AUTHORS INFORMATION

1 Tabriz Research Center of Infectious and Tropical Diseases, Tabriz University of Medical Sciences, Tabriz, Iran.

2 Center of Diseases Management Ministry of Health, Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

3 Assistant Professor, Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

4 Hematology, Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

5 RDCC Center of Medical School, Tabriz University of Medical Sciences, Tabriz, Iran.

ARTICLE INFORMATION

Hormozgan Medical Journal: 13 (3); e88765
Published Online: January 11, 2009
Article Type: Research Article
Received: April 17, 2007
Accepted: January 11, 2009

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Abstract

Introduction: Examination of Geimsa stained blood smears is the main method of
malaria diagnosis in our country, however, accuracy of results is largely depended on the
skill and laboratory conditions. This study was designed and carried out to assess the
current situation and to determine the effectiveness and quality improvement of malaria
diagnosis program.
Methods: In this analytical study, 100 positive blood samples for malaria by microscopic
method (85 Plasmodium vivax and 15 Plasmodium falciparum) and 15 negative samples were
collected from malarious areas of Iran and processed by Nested PCR to amplify the ssrRNA
(small sub-unit ribosomal RNA). Kappa agreement coefficient was calculated by SPSS
software.
Results: Except for one case, diagnosis either by microscopic or Nested-PCR was the same.
The exception was for one case diagnosed Plasmodium falciparum by microscopic and in
agreement method whiteas mix infection (Plasmodium vivax and Plasmodium falciparum) by
Nested-PCR. Agreement coefficient of microscopic and Nested-PCR methods was high.
Conclusion: In contrast to previous studies, high agreement coefficient of microscopic and
Nested-PCR methods in this study indicates, the effectiveness of quality improvement of
malaria diagnosis program in Iran.

Keywords

Malaria – Plasmodium Falciparum - Plasmodium Vivax – Polymerase Chain Reaction (PCR) - Iran

© 2009, Hormozgan Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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