Mass rearing of anopheles stephensi on human blood by artificial feeding under laboratory conditions


Hassan Nasirian 1 , * , Hossein Ladonni 2 , Abbas Poudat 3


1 Master of Medical Entomology & Vector Control, Department of Medical Entomology & Vector Control, Tehran University of Medical Sciences, Tehran, Iran.

2 Professor, Department of Medical Entomology & Vector Control, Tehran University of Medical Sciences, Tehran, Iran.

3 Instructor Department of Medical Entomology & Vector Control, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.


Hormozgan Medical Journal: 12 (3); e88955
Published Online: May 01, 2008
Article Type: Research Article
Received: June 19, 2005
Accepted: May 01, 2008




Introduction: In order to control the diseases that are transmitted to human and
animal by arthropod vectors, it is necessary to investigate vectors and to
recognize control methods as well. These studies are applicable by rearing the
colonies of arthropod blood-feeders like malaria and leishmaniasis in laboratory.
The purpose of this study was to produce mass production of Anopheles stephensi
(Lis.) by artificial feeding with human blood in insectary.
Methods: We prepared an apparatus composed of a digital thermostat with
sensor, element, contactor and a magnet in order to blood-feed An. stephensi.
The Para film “M” and a human whole blood unit were used as a membrane and
nutrient source for artificial feeding, respectively. The blood was placed in a soft
lid and its surface was covered with Para film “M”. The soft drink lids with
blood were placed on the cage surface and indirectly warmed by element. An.
stephensi fed from blood.
Results: Three consecutive generations were reared without blood-feeding on
host alive from the egg of mother colony. Female mosquito feeding rate was
47.7% to 64%. The average females feeding and ovipositing times were 10 and 9
times, respectively. No mortality was seen among the larvae. All of the pupae
emerged to the adult. The second and third generation of population ratios to first
population generation was 6 and 25 folds, respectively.
Conclusion: Our findings showed that artificial feeding by human blood,
especially in the investigational projects was completely efficient and suitable for
increasing mosquitoes’ population in specific time.


Artificial Feeding - Human Blood - Anopheles Stephensi - Mass Production

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