Pro oxidant and antioxidant status in patients with coronary artery disease proven angiographic ally


Kamal Khademwatan 1 , Fariborz Haghparast 2 , Ebrahim Eftekhar 3 , Jafar Nourooz-Zadeh 4 , *


1 Assitant Professor Department of Internal Medicine, Urmia University of Medical Science, Urmia, Iran.

2 Master of Biochemistry Islamic Azad University of Larestan, Larestan, Iran.

3 Master of Biochemistry, Urmia University of Medical Science, Urmia, Iran.

4 Professor Department of Biochemistry, Urmia University of Medical Science, Urmia, Iran.


Hormozgan Medical Journal: 12 (4); e89048
Published Online: October 11, 2008
Article Type: Research Article
Received: June 03, 2007
Accepted: October 11, 2008




Introduction: Coronary artery disease (CAD) is the major cause of death in both
industrial and developing countries. Evidences suggest that increase in free
radical generation and/or attenuated antioxidant defenses may be involved in the
pathogenesis of CAD. The aim of this investigation was to evaluate the plasma
measures of oxidative stress in patients with coronary artery disease documented
by angiography.
Methods: In this study case-control study, fifty eight patients with angiographic
ally proven coronary artery disease as cases and fifty five control subjects (Age
range: 41-70 years and 39-71 years, respectively) were recruited from heart
centre at Talaghani hospital affiliated with Urmia university of medical science.
Plasma vitamin E concentration was determined by high-performance liquid
chromatography. Plasma glutathione and malondialdehyde were studied
spectrophotometrically. Data analyses were performed by SPSS-11 statistical
software, using t-test.
Results:: Plasma malondialdehyde levels were markedly higher in the patients group
than in the controls (114.93±40.65 μmol/L versus 50.49±17.52 μmol/L; P<0.05).
Both vitamin E or glutathione levels were significantly lower in patient group
compared with controls (28.55±6.3 μmol/L versus 32.07±7.4 μmol/L; P<0.05 and
43.01±48 nmol/L versus 124.4±63.4 nmol/L; P<0.05 respectively). Significant
inverse correlations were seen between malondialdehyde levels and either vitamin E
or glutathione concentrations (P<0/05).
Conclusion: Our finding of impaired balance between antioxidant/pro oxidant
suggest that enhanced oxidative stress may be involved in the pathogenesis of
CAD. Understanding of the event that contribute to these changes, may provide
new opportunities for rational therapeutic strategies.


Coronary artery disease - oxidative stress - vitamin E - glutathione malondialdehyde

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