The effect of endurance training on dynein motor protein expression in Wistar male rats sciatic nerves with diabetic neuropathy

AUTHORS

Ali Khazani 1 , Reza Gharakhanlou 2 , * , Mohamad Reza Kordi 3 , Mansoureh Movahedin 4 , Shiva Jahani Golbar 5 , Masoud Rahmati 6

1 Department of Sport Sciences,Faculty of Humanities,Young Researchers and Elite Club,Ardabil Branch,Islamic Azad University,Ardabil,Iran.

2 Department of Physical Education and Sport Sciences,Faculty of Humanities,Tarbiat Modares University,Tehran,Iran.

3 Department of Physical Education and Sport Sciences,Faculty of Humanities,Tehran University,Tehran,Iran.

4 Department of Anatomical Sciences,Faculty of Medical Sciences,Tarbiat Modares University,Tehran,Iran.

5 Department of Physical Education and Sport Sciences,International Unit of University of Shiraz,Shiraz,Iran.

6 Department of Physical Education and Sport Sciences,Faculty of Humanities,Lorestan University,Khoramabad,Iran.

How to Cite: Khazani A , Gharakhanlou R , Kordi M R , Movahedin M , Jahani Golbar S, et al. The effect of endurance training on dynein motor protein expression in Wistar male rats sciatic nerves with diabetic neuropathy, Hormozgan Med J. 2018 ; 21(1):e87263. doi: 10.18869/acadpub.hmj.21.1.10.

ARTICLE INFORMATION

Hormozgan Medical Journal: 21 (1); e87263
Published Online: February 24, 2014
Article Type: Research Article
Received: November 12, 2013
Accepted: February 24, 2014
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Abstract

Introduction: Most neurodegenerative diseases are associated with the disruption of
axonal transport and this might also be related to diabetes-associated disorders affecting
the nervous system. Cytoplasmic dynein is a very important motor driving the movement
of a wide range of cargoes toward the minus ends of microtubules. The effects of
endurance training on dynein motor protein expression in Wistar male rats sciatic nerves
with diabetic neuropathy were investigated.
Methods: Twenty one male Wistar rats were divided into three groups: diabetic control
(DC: n=7), diabetic trained (DT: n=7) and Healthy control (HC: n=7). Two weeks post
STZ injection (45 mg/kg, i.p.), rats were subjected to treadmill exercise for 5 days a week
for over 6 weeks. Levels of mRNA and protein content were determined by Real time-
PCR and ELISA.
Results: The blood glucose levels and dynein mRNA in both sensory and motor parts
and dynein content in sciatic nerves were increased by diabetes (P≤0.05). Furthermore,
exercise training modulated the dynein mRNA and dynein content to normal levels in
diabetic trained rats (P≤0.05). Moreover, exercise training reduced blood glucose levels
in the DT rats (P≤0.05).
Conclusion: The results of the present study confirm that dynein expresses up-regulation
in STZ-induced diabetic rats. Increase in the amount of dynein expression can be a
compensatory response in the lower trophic support and burden imposed on autophagy
system. These alterations suggest that retrograde axonal transport may be impaired in
diabetic rats. In addition, endurance training as a non-pharmacotherapy strategy can
modulate these alterations.

Keywords

Diabetic Neuropathy Motor Proteins Dynein Motor Proteins Gene Espression

© 2018, Hormozgan Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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