The role of L-arginine and aerobic exercise in experimental renal ischemia reperfusion injury in male and female rats

AUTHORS

Effatsadat Vafamand 1 , Lotfali Bolboli 2 , Ardeshir Talebi 1 , Mehdi Nematbakhsh 3 , *

1 Water & Electrolytes Research Center,Isfahan University of Medical Sciences,Isfahan,Iran.

2 Department of Physical Education & Sport Sciences,University of Mohaghegh Ardebili,Ardebil,Iran.

3 Department of Physiology,Water & Electrolytes Research Center,Isfahan University of Medical Sciences,Isfahan,Iran.

How to Cite: Vafamand E , Bolboli L , Talebi A, Nematbakhsh M . The role of L-arginine and aerobic exercise in experimental renal ischemia reperfusion injury in male and female rats, Hormozgan Med J. 2017 ; 21(1):e87265. doi: 10.18869/acadpub.hmj.21.1.20.

ARTICLE INFORMATION

Hormozgan Medical Journal: 21 (1); e87265
Published Online: July 13, 2017
Article Type: Research Article
Received: May 02, 2017
Accepted: July 13, 2017
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Abstract

Introduction: Renal ischemia/reperfusion (I/R) injury due to reactive oxygen species
(ROS) formation is the main cause of acute kidney damage. Nitric oxide (NO)
biosynthesis and oxidative stress are closely related to the pathogenesis of renal I/R injury.
This study was undertaken to determine the effects of L-arginine (L-arg) as NO donor and
aerobic exercise (EX) and also the combination of L-arg with EX on renal I/R injury in
male and female rats.
Methods: 54 male and female Wistar rats were divided into four groups in each gender
as control, L-arginine (L-arg), treadmill exercise (EX), and L-arginine plus exercise (Larg
& EX). After 8 weeks of EX, animals were exposed to 45 min of bilateral kidney
ischemia followed by 24 hours of reperfusion. We assessed serum creatinine (Cr), Kidney
tissue damage score (KTDS), kidney weight (KW), serum nitrite and malondialdehyde
(MDA) levels.
Results: L-arg, EX or combination of L-arg &EX caused a significant decrease in serum
level of creatinine (Cr) (P<0.05). KTDS decreased significantly in all female treated
groups and male L-arg treated group (P<0.05) when compared with the control group.
However, the kidney weight was decreased significantly in male rats (P<0.05) at 24 hr
post reperfusion, and such observation was not seen in female. No significant differences
were detected in serum nitrite levels between the groups, but combination of L-arg &EX
decreased the serum level of malondialdehyde (MDA) in female significantly (P<0.05).
Conclusion: Pretreatment with L-arg seems to have protective effects against renal I/R
injury. The protective effect of exercise against renal I/R injury seems to be less than Larg
but the reno-protective effect of EX increases when combined with L-arg.

Keywords

Reperfusion L-arginine Aerobic Exercise

© 2017, Hormozgan Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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