The Role of Mas Receptor and Bradykinin on Nitric Oxide Production Response to Angiotensin 1 - 7 in Ovariectomized Rats Treated with Estradio

AUTHORS

Shadan Saberi 1 , Mehdi Nematbakhsh 2 , aghdas dehghani 3 , *

1 Department of Physiology, Faculty of Medicine, Ke rman University of Medical Sciences, Kerman, Iran.

2 Department of Physiology, Faculty of Medicine, Water & Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

3 Depa rtment of Physiology, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

How to Cite: Saberi S , Nematbakhsh M , dehghani A . The Role of Mas Receptor and Bradykinin on Nitric Oxide Production Response to Angiotensin 1 - 7 in Ovariectomized Rats Treated with Estradio, Hormozgan Med J. 2018 ; 22(1):e87287. doi: 10.29252/hmj.22.1.12.

ARTICLE INFORMATION

Hormozgan Medical Journal: 22 (1); e87287
Published Online: January 31, 2018
Article Type: Research Article
Received: November 14, 2017
Accepted: January 31, 2018
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Abstract

Introduction: The angiotensin 1-7 (Ang1-7) Mas receptor (MasR) axis, bradykinin (BK) and female sex hormone are involved in releasing of vasodilatory biomarkers including Nitric Oxide (NO). We examined the role of MasR and BK on NO metabolite (nitrite) production response to Ang 1-7 infusion in ovariectomized rats treated with estradiol.
Methods: A total of 48 female Wistar rats were divided into 2 main groups; ovariectomized treated with placebo (OVX) and ovariectomized treated with estradiol valerate (OVE) for period of two weeks. Then after anesthetization, the animals of each groups were divided into four subgroups that received MasR antagonist (A779) or , BK, BK+A779 or vehicle, and they were subjected to  Ang1-7 infusion (0, 100, 300 and 1000 ng/kg/min). The level of nitrite (NO metabolite) was measured by Griess method.
Results: The serum level of nitrite response to Ang 1-7 administration in OVE group was incrased when compared with OVX group, however when MasR was blocked by A799, the increased nitrite level was abolished. BK also increased the level of nitrite but co administration of BK and A779 did not enhance the nitrite level in both OVE and OVX groups.
Conclusion: Estradiol and Bk increase nitrite production in response to Ang 1-7 infusion in condition of MasR presence.

Keywords

Angiotensin 1-7 Mas Receptor Nitric Oxide Bradykinin Rat

© 2018, Hormozgan Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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