Molecular epidemiology of carbapenem resistance in Acinetobacter baumannii isolates in Shahid Mohammadi Hospital, Bandar Abbas, Iran

AUTHORS

Shahin Najar Peerayeh 1 , Afsaneh Karmostaji 2 , * , Maryam Ansari 3 , Sedigheh Javadpour 4 , Parivash Davoodian 5 , Nahid Moradi 6 , Mahshid Vahdani 7

1 Associate Professor Department of Medical Bacteriology, Tarbiat Modares University, Tehran, Iran.

2 Assistant Professor Department of Medical Bactriology, Hormozgan University of Medical Sciences, Banadr Abbas, Iran.

3 MSc of Molecular Biology, Infectious & Tropical Diseases Research Center , Hormozgan University of Medical Sciences, Banadr Abbas, Iran.

4 Associate Professor Department of Medical Bactriology, Infectious & Tropical Diseases Research Center, Hormozgan University of Medical Sciences, Banadr Abbas, Iran.

5 Assistant Professor Department of Infectious Diesease, Infectious & Tropical Diseases Research Center, Hormozgan University of Medical Sciences, Banadr Abbas, Iran.

6 MSc of Bacteriology, Infectious & Tropical Diseases Research Center, Hormozgan University of Medical Sciences, Banadr Abbas, Iran.

7 MSc of Molecular Biology, Infectious & Tropical Diseases Research Center, Hormozgan University of Medical Sciences, Banadr Abbas, Iran.

How to Cite: Najar Peerayeh S , Karmostaji A, Ansari M , Javadpour S, Davoodian P, et al. Molecular epidemiology of carbapenem resistance in Acinetobacter baumannii isolates in Shahid Mohammadi Hospital, Bandar Abbas, Iran, Hormozgan Med J. 2015 ; 19(1):e87376.

ARTICLE INFORMATION

Hormozgan Medical Journal: 19 (1); e87376
Published Online: May 18, 2014
Article Type: Research Article
Received: December 22, 2013
Accepted: May 18, 2014

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Abstract

Introduction: Acinetobacter baumannii is a major cause of nosocomial infections which
affect mainly disabled patients in intensive care units. The bacteria may acquire resistance to
antibiotics and hence can seriously endanger antibiotic therapy. The most important problem
facing treatment of A. baumannii is increasing reports of resistance to a wide range of
antibiotics, including carbapenems, as the treatment of choice for this bacterial infection.
Oxacillinase-type carbapenemases belonging to Class D beta-lactamases (OXA-type) are
among the main mechanisms of reasons for resistance to carbapenems. The present study
aimed to evaluate the patterns of antibiotic resistance and the prevalence of carbapenemase
genes of oxacillinases in clinical isolates of A. baumannii in a hospital in Bandar Abbas, Iran.
Methods: A total of 69 isolates of Acinetobacter were collected within two years from
different samples of patients’ bodies in the Shahid Mohammadi Hospital of Bandar Abbas.
The isolates genus was identified using biochemical methods and A. baumannii species
using PCR. Antibiotic resistance to imipenem and meropenem was identified through disk
diffusion method. OXA-type carbapenem resistance genes were identified by multiplex
PCR. The data were statistically analyzed through the chi-square test using SPSS 17, and
the graphs were plotted using Excel.
Results: Out of 69 Acinetobacter isolates, 57 (82.6%) had blaOXA-51 gene and were identified as
A. baumannii. Antibiogram showed a significant resistance to beta-lactams and other antibiotics
studied. The resistance percentage of the isolates to imipenem and meropenem antibiotics were
29.8% and 70.2%, respectively. Although all isolates were susceptible to colistin and polymyxin
B, 78.9% of isolates had blaoxa-23, 8.8% blaOXA-24, and 1.7% blaOXA-58 genes.
Conclusion: This study showed that beta-lactamase OXA-23 gene is the common
identified known carbapenemase among carbapenem-resistant A. baumannii in Bandar
Abbas Hospital. Evaluation of antibiotic-resistant genes in A. baumannii is necessary to
further control dissemination of antibiotic resistance genes.

Keywords

Acinetobacter baumannii – Carbapenemase – OXA-58 beta-lactmase

© 2015, Hormozgan Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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