Isolation and Identification of L-Asparaginase from Actinomycetes of Persian Gulf

AUTHORS

Fatemeh Izadpanah qeshmi 1 , Sedigheh Javadpour 2 , * , Kianoosh MalekZadeh 3 , Saeid Tamadoni Jahromi 4 , Mahsa Rahimzadeh 5

1 MSc Student of Microbiology, Jahrom Branch, Azad University, Jahrom, Iran.

2 Associate Professor Department of Microbiology, Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

3 Assistant Professor Department of Genetics, Hormozgan University of Medical Sciences, Molecular Medicine Research Center, Bandar Abbas, Iran.

4 PhD of Biotechnology, Persian Gulf and Oman Ecological Research Instituted, Bandar Abbas.

5 Professor Department of Biochemistry, Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.n.

How to Cite: Izadpanah qeshmi F, Javadpour S, MalekZadeh K, Tamadoni Jahromi S, Rahimzadeh M. Isolation and Identification of L-Asparaginase from Actinomycetes of Persian Gulf, Hormozgan Med J. 2014 ; 18(2):e87813.

ARTICLE INFORMATION

Hormozgan Medical Journal: 18 (2); e87813
Published Online: September 15, 2013
Article Type: Research Article
Received: April 20, 2013
Accepted: September 15, 2013

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Abstract

Introduction: L-asparaginase is an anti-neoblastic agent used in the chemotherapy of
lymphoblastic leukemia. Although this enzyme is widely distributed among microorganisms,
plants and animals, microorganisms have proved to be a better alternative, thus facilitating its
large-scale production. Actinomycetes are filamentous, Gram-positive bacteria widely
distributed in the marine environment. The aim of this study was isolation and molecular
identification of L-asparaginase producing actinomycetes isolated from Persian Gulf.
Methods: In this experimental study, 60 samples were collected from Persian Gulf in
Hormozgan, Iran. All colonieses were screened for L-asparaginase activity with modified M9
medium. L-asparaginase activity was measured by colorimertic method. The strain with high
L-asparaginase activity were selected and identified by nucleotide sequencing of 16S rRNA
gene.
Results: Thirty-two colonies of actinomycetes were obtained from sediment and seawater
samples, among which 23 isolates were L-asparaginase producers. The strain PG08 with
37×10-2 IU activity showed the highest activity. Based on the analysis 16S rRNA gene
sequences of this strain was showed maximum similarity to Streptomyces spp.OS1-33.
Conclusion: Actinomycetes isolated from Persian Gulf may be potential source of enzyme
L-asparaginase, Streptomyces spp. PG08 with high productivity L-asparaginase can be used
for chemotherapy of acute lymphoblastic leukemia.

Keywords

Asparaginase - Actinomycetes - 16S rRNA - Persian Gulf

© 2014, Hormozgan Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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