The effect of date aqua extract and melatonin on diabetic neuropathy in STZinduced diabetic rats

AUTHORS

Mandana Jafari 1 , Naser Zangiabadi 2 , * , Haleh Tajadini 3 , Mohammad Shabani 4 , Vahid Sheibani 4 , Amirhosein Ghasemi 5 , Meysam Shahidi 5

1 BSc of Biology, Pharmaceuitcs Research Center, Kerman University of Medical Sciences, Kerman, Iran.

2 Assistant Professor Department of Neurology, Kerman University of Medical Sciences, Kerman, Iran.

3 Assisstant Professor Department of Physiology, Kerman University of Medical Sciences, Kerman, Iran.

4 Associate Professor Department of Physiology, Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.

5 General Practitioner, Kerman University of Medical Sciences, Kerman, Iran.

How to Cite: Jafari M, Zangiabadi N, Tajadini H, Shabani M, Sheibani V, et al. The effect of date aqua extract and melatonin on diabetic neuropathy in STZinduced diabetic rats, Hormozgan Med J. 2013 ; 17(4):e88184.

ARTICLE INFORMATION

Hormozgan Medical Journal: 17 (4); e88184
Published Online: June 13, 2012
Article Type: Research Article
Received: October 29, 2011
Accepted: June 13, 2012

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Abstract

Introduction: Diabetes mellitus is often associated with chronic neuropathy, a condition
which is due to the disturbance in the function of peripheral nervous system. Studying the
effects of antioxidants in relieving neuropathy is of a great clinical importance. The aim of
this study was to compare the effect of aqua extract of date and Melatonin in preventing
neuropathic hyperalgesia in Streptozotocin-induced diabetic rats.
Methods: In this experimental study, 40 male Sprague dawley rats weighing 200-250gr
were divided into five groups of control, sham, diabetics+ melatonin and diabetics+ date
extract and diabetic+solvent. Diabetes was induced by intraperitoneal injection of
streptozotocin (45mg/kg B.W) solved in 0.05M citrate buffer. After confirming diabetes
statue, experimental groups received either melatonin (10mg/kg/day), date extract
(4mg/kg/day) or solvent for a duration of 6 weeks. At the end of the 6th week, all groups
were investigated using Hot plate and Tail flick tests. Analysis of variance with significance
level of P<0.05 was used for statistical analysis.
Results: In Hot plate test, solvent group showed significant decrease in the time of thermal
pain response compared to the non diabetic control group (P<0.01). Date extract group
showed an increase in the time of response to thermal pain as compared to the solvent group
(P<0.01) in a way that response time was close to that in the non diabetic control group.
Although time of response to pain in melatonin-administered group was close to that in the
non diabetic control group, melatonin group had no significant difference with other groups.
There was no significant difference among groups based on the results of Tail flick test.
Conclusion: The results of this study show that both date aqua extract and Melatonin as
antioxidants prevent and decrease neuropathic hyperalgesia in diabetic rats. In addition, the
protective effect of date aqua extract in prevention of diabetic hyperalgesia is more than
Melatonin.

Keywords

Melatonin – Diabetes – Neuropathy – hyperalgesia

© 2013, Hormozgan Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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