Effects of methotrexate administration on testis histomorphometrical features of rats

AUTHORS

Mirhadi Khayat Nouri 1 , * , Seyed Esmaeil Safavi 2 , Hamed Serati Nouri 3 , Arash Khaki 4

1 Assistant Professor Department of Pharmacology, Islamic Azad University Tabriz Branch, Tabriz, Iran.

2 Assistant Professor Department of Histology, Islamic Azad University Tabriz Branch, Tabriz, Iran.

3 Veterinarian, Islamic Azad University Tabriz Branch, Tabriz, Iran.

4 Assistant Professor Department of Pathology, Islamic Azad University Tabriz Branch, Tabriz, Iran.

How to Cite: Khayat Nouri M, Safavi S E, Serati Nouri H, Khaki A. Effects of methotrexate administration on testis histomorphometrical features of rats, Hormozgan Med J. 2010 ; 13(4):e88782.

ARTICLE INFORMATION

Hormozgan Medical Journal: 13 (4); e88782
Published Online: January 11, 2009
Article Type: Research Article
Received: July 17, 2008
Accepted: January 11, 2009

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Abstract

Introduction: Methotrexate (MTX) is a chemotherapeutic agent that used for the
treatment of a variety of tumors and inflammatory diseases. Methotrexate cause defective
cogenesis, spermatogenesis, fertility impairment and different pathological changes on
testis laboratory animals. The aim of this study was to investigate the effect of
methotrexate administration on morphometerical changes in the testis of adult rat.
Methods: In this experimental study 40 male wistar rats were selected and randomly
divided into four groups. Experiment groups were received 1mg/kg MTX (IP) per week
for two and four consequence week, respectively. However, control groups just received
normal saline (IP) for 14 and 28 consequence days, respectively. The testis were sampled
after passing of above times and stained by H & E technique. Then diameter of
seminiferous tubules, thickness of interstitial tissue, and thickness of testicular capsule,
were studied. Data were analyzed by analysis of variance.
Results: Our study showed that methotrexate had destructive effects on testis tissue
and spermatogenesis in test group compared to control group. Results showed that
within 28 days after methotrexate administration diameter of seminiferous tubules
and thickness of epithelium of seminiferous tubules significantly decreased
(P<0.05), but thickness of interstitial tissue and thickness of testicular significant
increased (P<0.05) compared to controls.
Conclusion: Results showed that methotrexate administration causes decline in
spermatogenesis and significant changes in diameter of seminiferous tubules, thickness of
epithelium of seminiferous tubules, thickness of interstitial tissue, and thickness of
testicular capsule in rat testis tissue. These changes may via the reducing of cell divide and
toxic effects of methtrexate.

Keywords

Methotrexate – Testis – Rats Wistar

© 2010, Hormozgan Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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