Effect of insulin and L-ascorbic acid on rate of neuronal apoptosis in dentate gyrus and CA3 region of hippocampus in type 1 diabetic rats

AUTHORS

Shahriyar Ahmadpour 1 , * , Yousef Sadeghi 2 , Mehrnoosh Sheybanifar 1 , Hossein Haghir 3

1 Assistant Professor Department of Anatomy, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

2 Professor Department of Anatomy, Shaheed Beheshti University of Medical Sciences, Tehran, Iran.

3 Associate Professor Department of Anatomy, Mashhad University of Medical Sciences, Mashhad, Iran.

How to Cite: Ahmadpour S, Sadeghi Y, Sheybanifar M, Haghir H. Effect of insulin and L-ascorbic acid on rate of neuronal apoptosis in dentate gyrus and CA3 region of hippocampus in type 1 diabetic rats, Hormozgan Med J. 2010 ; 13(4):e88785.

ARTICLE INFORMATION

Hormozgan Medical Journal: 13 (4); e88785
Published Online: October 31, 2009
Article Type: Research Article
Received: May 24, 2009
Accepted: October 31, 2009

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Abstract

Introduction: The aim of this study was to determine the effects of Insulin and Lascorbic
acid on prevention of neuronal in granular layer of dentate gyrus and
pyramidal layer of CA3 of hippocampus in streptozotocin-Induced diabetic rats.
Methods: In this experimental study, diabetes type 1 was included by a single
intraperitoneal injection of streptozotocin (STZ) (60mg/kg) in male adult wistar rats. After
six weeks, STZ-injected rate were divided randomly into four groups as follow: (1)
treated with Insulin (2) treated with Insulin + ascorbic acid (3) treated with ascorbic acid,
and (4) untreated diabetic. After two weeks the brains were removed. Numerical density
of TUNEL positive neurons in granular layer of the dentate gyrus and pyramidal layer
were counted. Ultra structure of the injured neurons was studied.
Results: Our findings showed diabetes type 1 caused Increased numerical density of
TUNEL positive neurons in the dentate gyrus, pyramidal layer of CA3. Ultra
structure showed server chromatin condensation, cytoplasmic radio-opacity and
preserved nuclear membrane integrity indicative of apoptosis. In treated diabetic
groups with Insulin, Insulin + ascorbic acid and ascorbic acid, numerical density of
TUNEL positive neurons in the granular layer of the dentate gyrus and pyramidal
layer of CA3 were lower than those of untreated group (P<0.001).
Conclusion: It seems that, treatment of diabetic rats with insulin, ascorbic acid and
combined treatment could prevent neuronal apoptosis.

Keywords

Diabetes Mellitus – Streptozocin – Apoptosis – Insulin – Ascorbic Acid

© 2010, Hormozgan Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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